Relatively high signal was observed for constructs transcribed from Pol-I promoters, known to result in nucleolar localization of the RNA ( 50 , 51 ), whereas a substantially lower—and yet highly significant—signal was observed for those transcribed from constructs driven by Pol-II promoters (median 17.4% and 2.4%, respectively).
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A systematic dissection of determinants and consequences of snoRNA-guided pseudouridylation of human mRNA.
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