Molecular Signature of Neuroinflammation Induced in Cytokine-Stimulated Human Cortical Spheroids.

The RNA-seq data was validated by qPCR analysis of a number of neuroinflammation-related genes modulated in the stimulated hCSs ( Supplementary Figure S3 ), showing at all time points a highly significant correlation between the RNA-seq and qPCR data from the TNFα-stimulated hCSs ( Supplementary Table S5 ) as well as between the RNA-seq and qPCR data from the IL-1β-stimulated hCSs ( Supplementary Table S6 ).

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