Oral vaccination with recombinant Lactobacillus casei expressing Aha1 fused with CTB as an adjuvant against Aeromonas veronii in common carp (Cyprinus carpio).

9 , TNF-α expression in the spleen, head kidney and intestine of the recombinant pPG-Aha1/Lc CC16 and pPG-Aha1-CTB/Lc CC16 groups was increased rapidly at the early stage of immunization, with a decreasing trend at day 28, and reached the highest level at the second booster immunization ( p < 0.05).

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casei groups showed an increasing trend and reached the peak at the last booster immunization, which was significantly higher than the PBS group ( p < 0.05).

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