To further explore whether FUT3-AS1 or histone H4 acetylation regulates the binding activity of Sp1 to the FUT3 core promoter, we also performed a dual-luciferase assay and found that the transcriptional binding activity of Sp1 showed an extremely significant decline in siFUT3-AS1 IPEC-J2 cells, and significantly increased in TSA treated IPEC-J2 cells ( Fig 3M ).
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Insight into mechanisms of pig lncRNA FUT3-AS1 regulating E. coli F18-bacterial diarrhea.
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