OPN5 Regulating Mechanism of Follicle Development Through the TSH-DIO2/DIO3 Pathway in Mountain Ducks Under Different Photoperiods.

Detection of TSH-DIO2/DIO3 pathway proteins revealed that the results of THR and DIO2 western blots ( Figures 5G–I ) and the immunohistochemical results of TSHβ ( Figure 5J–M ) demonstrated an increasing trend under long-day photoperiod and a down-regulation of TSH, THR, and DIO2 protein expression under short-day photoperiod, which were positively correlated with the trend of OPN5 protein expression. 3.5 Expression Levels of the Main Factors of the HPG Axis The results of the expression of genes related to the HPG axis found that the relative gene expression of GnRH , GnRHR , FSH , LH , VIP, and PRL gradually increased in group L, and the relative gene expression of GnIH and GnIHR gradually decreased; the opposite of group S ( Figures 6A–H ).

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