The mCherry-AHA1 (acceptor) fluorescence excited by 488 nm light (donor) gave strong and highly significant signals when co-expressed with GFP-SYP132 but FRET signal was significantly lower when mCherry-AHA1 was co-expressed with GFP on its own or with GFP-ΔNH abc -SYP132 ( Figure 6C ).
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SNARE SYP132 mediates divergent traffic of plasma membrane H+-ATPase AHA1 and antimicrobial PR1 during bacterial pathogenesis.
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