Naïve Primary Mouse CD8⁺ T Cells Retain <i>In Vivo</i> Immune Responsiveness After Electroporation-Based CRISPR/Cas9 Genetic Engineering.

Although there was a weak trend on day 7 that OT-I cells nucleofected using P3 buffer outperformed other groups ( Figure 2B ), none of the nucleofected OT-I cell populations displayed a statistically significant difference from NTC OT-I cells, except that no RNP control in the spleen was slightly less abundant.

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