Optimization of the composition of a solid culture medium for Mycobacterium avium subsp. paratuberculosis using factorial design and response surface methodology.

RSM design – Model fitting and validation The results of ANOVA indicated that the models for all responses were highly significant ( p < 0.0001).

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Of these six factors, five also had a significant effect on R 2 as shown in Figure 2B (A, B and F p < 0.0001; AF, p value 0.0022 and ABF, p value 0.0004), while factor BF was almost significant ( p value 0.055).

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