Implementation of a Design of Experiments to Improve Periplasmic Yield of Functional ScFv Antibodies in a Phage Display Platform.

21 However,IPTG concentration (A) in the range of 0.05-1 mM was considered as a non-significant linear termbecause increasing the concentration of IPTG did not produce an increasing trend in the scFv yield ( Figure 2d ).

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