In patients from the Burkina Faso and Mozambique cohorts, the same trend was observed, but the correlation was weaker and marginally statistically significant in Burkina Faso ( r = -0.38, p = 0.040) and not statistically significant in Mozambique ( r = -0.24).
Indeed, in a published large multicentre study in South-East Asia, 82 the pfap2-g / gexp02 transcripts ratio showed a weak to moderate but statistically highly significant negative correlation with parasite age statistically estimated from the full transcriptomes, in six different study sites (average r between the sites -0.35, range -0.19 to -0.45, p < 0.05 in all sites) (Supplementary Figure 6).