Overexpression of <i>MsRCI2D</i> and <i>MsRCI2E</i> Enhances Salt Tolerance in Alfalfa (<i>Medicago sativa</i> L.) by Stabilizing Antioxidant Activity and Regulating Ion Homeostasis.

The expression trend of the SOS1 gene in the two transgenic alfalfa roots was similar, that is, it was significantly increased at 6 h and decreased at 12 h under salt stress, and the up-regulation of the SOS1 gene was extremely significant in MsRCI2D transgenic roots ( p < 0.01).

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In this study, the MsRCI2D,-E genes in alfalfa could respond to salt and alkali stress and be regulated by ABA; the expression levels of the MsRCI2D,-E genes in roots and leaves showed an overall trend first increasing and then decreasing, but the up-regulation of the MsRCI2E gene was more rapid than that of the MsRCI2D gene ( Figure 2 ).

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