Although the differences between five ‘good’ G4 X and non-G4 X were highly significant in both 0K and 20K buffer, this difference between average F value s ( ΔMedian) was actually higher in 20K (0.82) than in 0K (0.64), meaning that the discrimination between the two groups (G4 vs non-G4) is better in the presence of 20 mM potassium (20K).
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Iso-FRET: an isothermal competition assay to analyze quadruplex formation in vitro.
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