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A robust, low-cost instrument for real-time colorimetric isothermal nucleic acid amplification.

PLoS One · 2022 · PMC9524685 · PMID 36178899

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a weak trendno p-value reported
A weak trend in midpoint signal brightness is also observed across tube locations, with tubes at the edges of the block producing brighter signals than tubes at the center of the block.

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Quoted from the open-access full text in Europe PMC under the licence the publisher applied. The sentence is reproduced exactly as published; the emphasis is ours.