p < 0.05 was regarded as statistically significant, p < 0.01 was regarded as very significant, and p < 0.001 was regarded as extremely significant. 3 Result 3.1 Intestinal histological changes Histological analysis of the intestinal sections from the PBS-injected group and the DIV1-infected group are shown in Figure 1 .
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Integrated analysis of intestinal microbiota and metabolomic reveals that decapod iridescent virus 1 (DIV1) infection induces secondary bacterial infection and metabolic reprogramming in <i>Marsupenaeus japonicus</i>.
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