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Optimized Sample Preparation and Microscale Separation Methods for High-Sensitivity Analysis of Hydrophilic Peptides.

Molecules · 2022 · PMC9573374 · PMID 36235181

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a decreasing trendno p-value reported
The three shallower gradients (2step 4-20-40, Lin 4–27, and 2step 4-25-40 in increasing order of slope) showed a decreasing trend with increasing slope due to co-elution and ion suppression of some components.

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