Function of <i>Vitellogenin receptor</i> gene in reproductive regulation of <i>Zeugodacus cucurbitae</i> (Coquillett) after short-term high-temperature treatment.

The relative expression of all groups showed a decreasing trend and peaked at 24 h, and the maximum relative expression of NC, injury, and siRNA in the 45°C treatment was lower than that in the 25°C treatment ( Figure 4B ). 3.2.3 Effect of interfering with target genes on the expression of Vg-2 gene in Z. cucurbitae (Coquillett) Expression levels of the Vg-2 gene were analyzed by injecting siRNA into 5-day-old female adults and collecting samples after 24, 48, and 72 h.

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The relative expression of each group in the 45°C treatment showed an increasing trend and reached the peak at 72 h.

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