We derived the average fluorescence lifetime (τ avg ) and ORR of IFNγ-M1 and IL-4-M2 macrophages from the full FoV of 2P-FLIM data and observed an increasing trend of τ avg in response to the application metabolic enzymatic inhibitors.
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Non-invasive classification of macrophage polarisation by 2P-FLIM and machine learning.
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