Statistical analysis showed that the relative volume difference, compared to the fresh state, was already highly significant from the first day of staining with Lugol’s iodine, whereas, for Hf-WD POM staining, the relative volume difference reached statistical significance only after 11 days of staining (Supplementary Fig. 1 ).
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Cryogenic contrast-enhanced microCT enables nondestructive 3D quantitative histopathology of soft biological tissues.
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