Structural-Functional Relationship of the Ribonucleolytic Activity of aIF5A from <i>Sulfolobus solfataricus</i>.

Mixtures of N-His-aIF5A and rRNA were analyzed and the results, elaborated and presented in Figure 4 D, show a decreasing trend of the Rg as a function of the increasing protein:RNA ratio. rRNA is a substrate for the protein-cleaving activity, and therefore, we may hypothesize that increasing the amount of protein leads to degradation of most of the RNA molecules.

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