highly significantP < 0.001
Differences were considered significant when * P < 0.05, very significant when ** P < 0.01, and highly significant when *** P < 0.001 and **** P ≤ 0.0001.
Differences were considered significant when * P < 0.05, very significant when ** P < 0.01, and highly significant when *** P < 0.001 and **** P ≤ 0.0001.