Tea tree oil nanoliposomes: optimization, characterization, and antibacterial activity against Escherichia coli in vitro and in vivo.

After the quadratic regression fitting, the fitted equation of the relationship between the sealing rate Y and the influencing factor X was obtained: Y = + 79.74 + 0.1663 X 1 + 7.27 X 2 − 2.04 X 3 − 0.5700 X 1 + 5.57 X 1 X 3 − 0.2725 X 2 X 3 − 2.47 X 1 2 − 8.76 X 2 2 − 7.16 X 3 2 It can be seen that the model is highly significant ( P model < 0.0001), with a non-significant P = 0.0589 for the misfit term.

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P < 0.05 was considered as significant difference and P < 0.01 was extremely significant difference.

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