Energy acquisition strategy for reproduction in a semelparous squid.

Nine out of the 14 FAs showed significant differences in the relative amounts between maturity stages, where an increasing trend was found for 14:0 ( F = 20.41, p < 0.05), 20:2 ( F = 4.17, p = 0.006), 20:3n3 ( F = 3.08, p = 0.024), 20:4n6 ( F = 4.48, p = 0.004) and 20:5n3 ( F = 3.20, p = 0.028), a decreasing trend for 18:0 ( F = 4.31, p = 0.005), 18:1n9 ( F = 3.53, p = 0.013) and 20:1 ( F = 18.37, p < 0.05), and an increase for 16:0 from stages III–VI ( F = 18.18, p < 0.05), followed by a decrease at stage VII (Fig. 4 a; Additional file 1 : Table S1).

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Isotopic niche width showed an increasing trend with maturation, and isotopic niche space exhibited greater overlap at advanced maturity stages.

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