marginally significantp = 0.07
The number of ASVs varied widely among samples ranging from 5 to 125 ASVs per sample, with a marginally significant reduction of ASV richness in smokers compared with non-smokers (mean ± SEM: 55 ± 5 vs. 34 ± 5 ASVs/sample for smokers and non-smokers, respectively; ANOVA, p = 0.07) but no difference in richness between HIV- and HIV+ (46 ± 5 vs. 44 ± 5; ANOVA, p = 0.86).