Characterization of FcγRIa (CD64) as a Ligand Molecule for Site-Specific IgG1 Capture: A Side-By-Side Comparison with Protein A.

One-way analysis of variance, ANOVA, was used to reveal the statistically significant data ( p < 0.05 was considered significant and p < 0.005 was considered highly significant). 2.4 IgG1 Binding Capacity Analysis with Protein L-Captured Antibodies as Ligands: FcγRIa and Protein A were Used as Analytes The binding analyses of the FcγRIa and protein A (Sigma-Aldrich) in solution were carried out on a protein L-immobilized dextran-coated CM5 chip (Cytiva).

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