A New Cell Line Derived from the Spleen of the Japanese Flounder (<i>Paralichthys olivaceus</i>) and Its Application in Viral Study.

BIV and HIRRV infections showed highly significant ( p < 0.01), and IHNV infection showed extremely significant differences ( p < 0.001), but infection with LCDV showed no significant difference from the control group ( p > 0.05) ( Figure 9 B).

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JFSP cells could be maintained but not grown in 5% FBS at the same temperature, while the JFSP cell growth showed an increasing trend as the FBS content in the medium increased, with good growth in 15–30% FBS and cells reaching maximum growth on day 3 or 4 after culture.

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