For 42- to 140-bp IESs, we observed an even stronger sequence bias with an overrepresentation of the TACAG boundary among very early IESs, with the increase of the C frequency at the third position being highly significant (77% vs. 30% for very early vs. late IESs, respectively).
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Developmental timing of programmed DNA elimination in <i>Paramecium tetraurelia</i> recapitulates germline transposon evolutionary dynamics.
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