highly significantP < .05
And for gene expression study, least three independent parallel experiments were taken, organized, and calculated using Microsoft Excel 2010 and then analyzed using SPSS software (IBM SPSS Statistics, Version 20), p-values were calculated using independent samples t-test, p < .05 indicates significant, p < .01 indicates highly significant, and finally Plotting was performed using the software ORIGIN 2018 (asterisks indicate significant differences: *P < .05, **P < .01).