Optimized Design and Preparation of Ag Nanoparticle Multilayer SERS Substrates with Excellent Sensing Performance.

However, as can be seen from Figure 1 d, the LEF intensity at point A (in the gaps between adjacent AgNPs at the bottom layer, which is marked in Figure S1 ) has a decreasing trend when the number of layers exceeds two, mainly because the scattering effect of nanoparticles led to the incident light flux gradually decreasing from the top to the bottom, and the plasma coupling effect of the bottom AgNPs weakening [ 15 ].

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