Closing the gap to effective gene drive in Aedes aegypti by exploiting germline regulatory elements.

The sds3 G1-Cas9 was tested under conditions similar to the M and P crosses in the present study, but a marginally significant inheritance bias (67%, n = 176, Fisher’s two-sided exact test, p = 0.0495) of the w U6b-GDe element was shown only in one out of the four crossing regimes (F 0 Cas9-bearing female, F 1 trans-heterozygous female), whereas the same Cas9 isoline mediated significant bias of the kmo sgRNAs inheritance in all six combinations of parental crossing in our hands (Figs. 1 – 3 ).

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