First, h SkMs behavior in 3D culture was explored, as shown in Figure 5A h SkMs showed a significant upregulation of Pax3 and MYH2 at day 21 (258-fold and 261-fold, respectively, p < 0.0001), as well as MyoD1 , Myf5 , Myf6 , and Desmin genes with an increasing trend from day 0–21.
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Peripheral blood mononuclear cells contribute to myogenesis in a 3D bioengineered system of bone marrow mesenchymal stem cells and myoblasts.
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