Co-substrate composition is critical for enrichment of functional key species and for process efficiency during biogas production from cattle manure.

In reactor R3, higher diversity of microbial community indicated a positive influence of addition of potato starch and, among other families, DTU013 and Dysgonomonadaceae were highly significant ( p < 0.05) in both LFCa (>2) and LDa (>4) (Figures 3 , 4 ).

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Reactor R1 showed an increasing trend in VFA level, starting approximately after day 75 (15 days after introduction of co‐substrate) and increasing to a peak around 1.6 g L −1 at day 154 (Figure S3 ).

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