The positive Spearman correlation coefficient ( P = 0.033, R 2 = 0.586) between the qPCR and RNA-seq data was highly significant, indicating that our RNA-seq data were robust and could be used in subsequent analyses.
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Integrated single-molecule real-time sequencing and RNA sequencing reveal the molecular mechanisms of salt tolerance in a novel synthesized polyploid genetic bridge between maize and its wild relatives.
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