Assessing nuclear versus mitochondrial cell-free DNA (cfDNA) by qRT-PCR and droplet digital PCR using a piglet model of perinatal asphyxia.

Mol Biol Rep · 2023 · PMC9889441 · PMID 36512170

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an increasing trendno p-value reported

Applying the qRT-PCR method, ncfDNA concentrations in piglets exposed to hypoxia revealed an increasing trend from 7.1 ng/ml to 9.5 ng/ml for HK2 (hexokinase 2) and from 4.6 ng/ml to 7.9 ng/ml for β-globulin, respectively, whereas the control animals showed a more balanced profile.

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a decreasing trendno p-value reported

We observed a decreasing trend in the amount of cfDNA at the end of hypoxia compared to the baseline in the intervention group: 492.8 ng/ml to 347.9 ng/ml.

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Quoted from the open-access full text in Europe PMC under the licence the publisher applied. The sentence is reproduced exactly as published; the emphasis is ours.