Identification of thyroid hormone response genes in the remodeling of dorsal muscle during <i>Microhyla fissipes</i> metamorphosis.

Front Endocrinol (Lausanne) · 2023 · PMC9937655 · PMID 36817577

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Confirmation of the TH direct upregulated genes by qPCR To validate the TH directly regulated gene by RNA-seq analysis, 12 genes ( TRβ , TH/bZIP , PFASF , DUSP9 , CCN4 , CYP2K , SPG20 , SOX4 , BGLAP , CMKLR , TMLHE , and KLF9 ) with highly significant expression changes after TH and TH_CHX treatment were randomly selected for qRT-PCR analysis.

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