highly significantP < 0.01
Differences were considered to be statistically significant at * P < 0.05, and highly significant at ** P < 0.01, *** P < 0.001 and **** P < 0.0001.
Differences were considered to be statistically significant at * P < 0.05, and highly significant at ** P < 0.01, *** P < 0.001 and **** P < 0.0001.