New Insights into the Inhibition of Hesperetin on Polyphenol Oxidase: Inhibitory Kinetics, Binding Characteristics, Conformational Change and Computational Simulation.

Either continuously adding hesperetin into the PPO solution ( Figure 2 D) or continuously adding PPO into the hesperetin solution ( Figure 2 E), the added hesperetin did not react completely with PPO (and vice versa), so that free hesperetin or PPO existed in the system; the concentration profile of the PPO–hesperetin complex always showed an increasing trend.

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