A versatile multimodal chromatography strategy to rapidly purify protein nanostructures assembled in cell lysates.

J Nanobiotechnology · 2023 · PMC9960191 · PMID 36829140

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practically significantno p-value reported

We chose to work with CFP-SpyC, YFP-SpyC, and SpyC as model functional proteins due to the convenience of FRET assays to investigate filament composition, but γPFD-SpyT could be functionalized with any combination of practically significant proteins, including enzyme cascades [ 5 ], and purified in the same manner.

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