Ejaculatory Abstinence Affects the Sperm Quality in Normozoospermic Men-How Does the Seminal Bacteriome Respond?

[ 11 ] observed no significant changes in the proportion of spermatozoa with alterations to the DNA molecule, an increasing trend was observed in sperm DNA fragmentation with each recurrent semen collection at 2-h intervals after the first one, although no specimen trespassed acceptable levels (<29%) established for sperm DNA damage [ 50 ].

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Moreover, a decreasing trend was observed in these parameters at second, third, and fourth evaluations of semen collected following two hours of abstinence each when compared to the first assessment after 3–4 days of abstinence [ 16 ].

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