Effects of combined nitrogen and phosphorus application on protein fractions and nonstructural carbohydrate of alfalfa.

Under the same P treatment, the CP, SOLP, NDIP and NPN contents showed an increasing trend with the increase of N fertilization (in the 2.50 – 6.81, 3.48 – 9.70, 2.75 – 5.89, and 4.56 – 14.09% ranges, respectively, p < 0.05), while the ADIP content showed a decreasing trend (in the 0.56 –5.06% range, p > 0.05).

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Effects of nitrogen and phosphorus combined application on protein fractions The contents of PA, PB 1 , and PB 3 in the protein fractions showed an increasing trend and then decreased with an increase in P fertilization, while the contents of PB 2 and PC showed a trend of decreasing and then increasing P application ( Figure 2 ).

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