Although cytokine responses in SET1 and SET2 were not significantly different, SET2 showed a trend for reduced IFN-γ and IL-2 expression in CD4+ T cells as well as lower frequency of all three cytokines in CD8+ T cells ( Fig 2C ).
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A whole blood intracellular cytokine assay optimised for field site studies demonstrates polyfunctionality of CD4+ T cells in acute scrub typhus.
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