Production of diverse brGDGTs by Acidobacterium Solibacter usitatus in response to temperature, pH, and O₂ provides a culturing perspective on brGDGT proxies and biosynthesis.

MAF (months above freezing temperature) producing moderately significant slope/intercept differences (Δ slope = 0.006 ± 0.003°C −1 , Δ intercept = −0.17 ± 0.06, p <.05), and regression vs MAT producing highly significant differences (Δ slope = 0.011 ± 0.003°C −1 , Δintercept = −0.29 ± 0.06, p < .001) from the culture data (Table 1c ).

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