Role of the Extracytoplasmic Function Sigma Factor SigE in the Stringent Response of <i>Mycobacterium tuberculosis</i>.

( 6 ), in low-phosphate environments, an increased transcription of the genes encoding the two-component system SenX3-RegX3 (even if the increase of senX3 did not reach statistical significance) was observed in the WT.

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Ppk1 was also reported to be essential for the activation of the stringent response through the SigE-dependent induction of relA ( 5 , 6 ); indeed, in our experiment, relA expression showed an increasing trend starting from 6 h after exposure to the low-phosphate environment, even if this increase did not reach statistical significance (Data Set S2).

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