Although our attempts to use CD3 and/or CD8 densities to predict the response to neoadjuvant treatment did not reach statistical significance, our use of the IS B tool resulted in similar results to those previously published 7 .
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A type I interferon footprint in pre-operative biopsies is an independent biomarker that in combination with CD8<sup>+</sup> T cell quantification can improve the prediction of response to neoadjuvant treatment of rectal adenocarcinoma.
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