Changes in Absolute Cell Number of Dorsal Skin Tissue and ALN The absolute cell number of CD19+, CD4+, CD8+, CD4+/CD69+, and CD23+/B220+ for ALN showed a trend of significant increase in DNCB_CTL compared to NC/Nga_Nr ( ## p < 0.01 or ### p < 0.001 (compared with NC/Nga_Nr)).
Immunofluorescence Staining to Evaluate Claudin 1 and Sirt 1 Expression in Dorsal Skin Tissue The evaluation of Claudin 1 and Sirt 1 expression in dorsal skin tissue showed that the level of expression was reduced in DNCB_CTL compared to NC/Nga_Nr, while in DNCB_Dexa, the skin proteins showed an increasing trend in the level of expression compared to DNCB_CTL, although without statistical significance.