However, Post_EVs showed a trend in the increase of circulating CD31 + and CD106+ (p = 0.87) EVs, suggesting the activation of the vascular system and the release of EVs from endothelial cells, as well as increase in IB4+ and Irisin+ (p = 0.37 and p = 0.25 respectively) EVs, respectively released by microglia cells and muscles.
In accordance with the immunofluorescence results, we found that Post_EVs were able to significatively increase the transcription of SOD1 and HMOX1 genes, while only a positive trend was observed for CAT and NQO1 ( Supplementary Fig. 3 ). 3.4 Post_EVs protect cardiomyocytes from oxidative stress Since Post_EVs resulted to be able to activate protective and antioxidant pathways in cardiomyocytes, we decided to investigate their protective role in pro-oxidant conditions. hCM were treated with 150 μM of H 2 O 2 for 1hr.