SGK1 inhibition induces fetal hemoglobin expression and delays polymerization in sickle erythroid cells.

1 In small trials, metformin showed a trend to a modest increases in HbF in patients with SCD 17 , 18 and in a large retrospective study lower rates of vaso-occlusive crises and acute chest syndrome were found in patients with SCD who take metformin than in those who do not, however subset analysis failed to show a difference before and after metformin treatment. 19 We confirmed expression of SGK1 in CD34+ cells by small-cell RNA sequencing analysis from human erythroid cells showing that SGK1 expression increases during erythroid differentiation, as confirmed by western blotting ( Figure 1 A).

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