In the case of irf1b , the expression in cep290 was 1.9-fold higher than control, which was similar to what was identified by RNAseq (1.45-fold), but the qRT-PCR failed to reach statistical significance ( p = 0.11) due to high variability between samples.
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Comparative analysis of transcriptional changes in zebrafish <i>cep290</i> and <i>bbs2</i> mutants by RNA-seq reveals upregulation of inflammatory and stress-related pathways.
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