Analysis of RAS and drug induced homo- and heterodimerization of RAF and KSR1 proteins in living cells using split Nanoluc luciferase.

Also note the consistent and highly significant increase in Nluc activity in unstimulated KRAS G12V expressing cells compared to EGF stimulated cells transfected with pMIG e.V.

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In contrast, the E70K, the I71A/L78A double mutation and the R615H substitution showed a trend for reduced KSR1/BRAF association in the Nanoluc assays that became significant in the context of KRAS G12V and Sorafenib treatment (Fig. 6 B).

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