Due to the limitation of the CRISPR-Cas system to the domains of archaea and bacteria, the separation, optimization, and production of the CRISPR-Cas transfer carriers and vectors is quite significant for the implementation of RNA-guided nucleases which carry the ability to target new strains for instance, multi-drug resistant (MDR) pathogens and other crucial elements of the endogenous microbiota [ 57 ].
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CRISPR-Cas9 System: A Prospective Pathway toward Combatting Antibiotic Resistance.
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