approached the level of significancep = 0.0501
Of note, this trend approached the level of significance at M1, and achieved statistical significance between M2 and M5 (M1 p = 0.0501, M2 p = 0.0009, M3 p = 0.0496, M4 p = 0.0019, M5 p = 0.0312; Mann–Whitney U test).